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KMID : 0043320070300020191
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Archives of Pharmacal Research
2007 Volume.30 No. 2 p.191 ~ p.198
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Immunoregulatory Abnormalities of T Cells and Hyperreactivity of B Cells in the In Vitro Immune Response in Pristane-Induced Lupus Mice
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Chae Byeong-Suk
Shin Tae-Yong
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Abstract
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Systemic lupus erythematosus (SLE) is characterized by overactive B cells that differentiate into autoantibody-forming cells, aberrant T cell function that provides helping B cells produce autoantibodies, and overproduction of proinflammatory cytokines. However, immunodysregulation in lupus pathogenensis remains incomplete. We examined mitogen-stimulated production of proinflammatory cytokines, cell proliferation, T cell activation, and T cell apoptosis in vitro in pristane-induced lupus BALB/c mice compared to normal mice. LPS-stimulated production of IL-6 and IL-10 by splenocytes and macrophages from pristane-induced lupus mice were remarkably up-regulated compared to normal mice, whereas production of macrophage TNF-α was significantly down-regulated. Moreover, in vitro production of IL-2, IL-6, IL-10 and IFN-γ by Con A-stimulated splenocytes, cell proliferation in LPS- or Con A-stimulated- thymocytes and splenocytes, and expression of CD69+CD4+ T cells in Con A-stimulated splenocytes were greatly increased in cells derived from pristane-induced lupus mice compared to normal mice. In addition, splenic T cells and CD4+ T cells in thymocytes from pristane-induced lupus mice were more resistant than nonautoimmune normal cells to Con A-induced apoptosis. Our findings indicate that immunoregulatory abnormalities of T cells and hyperreactivity of B cells in the in vitro immune responses in pristane-induced lupus mice may explain some of lupus pathogenesis.
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KEYWORD
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IL-6, IL-10, IL-2, IFN-γ, TNF-α, Lupus, Pristane, T cell activation, T cell apoptosis
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